Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
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چکیده
منابع مشابه
Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris.
BACKGROUND Multiple cognate gene copy clones have often been used in order to increase the yield of recombinant protein expression in the yeast Pichia pastoris. The method of posttransformational vector amplification (PTVA) has allowed for the efficient generation of multi-copy clones in P. pastoris. However, despite its relative ease and success, this process can be expensive and time consumin...
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The methylotropic yeast, Pichia pastoris, has been successfully used for the expression of a wide variety of recombinant proteins (2,3). The expression level of many of these proteins is influenced by the number of copies of the gene of interest introduced into the P. pastoris genome (1). In the past few years, a system for the screening of multiple-copy inserts has become commercially availabl...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2016
ISSN: 1475-2859
DOI: 10.1186/s12934-016-0432-8